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s30 extract  (Promega)

 
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    Promega s30 extract
    S30 Extract, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s30+extract/e++coli+t7+s30+extract+system+for+circular+dna/us12351619-517-27-29
    Average 90 stars, based on 1 article reviews
    s30 extract - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Prostate specific membrane antigen binding fibronectin type III domains
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated at 30° C. with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL. .. After 1 hour, 375 μL of blocking solution (1×TBS pH 7.4, 0.01% I-block (Life Technologies, # T2015), 100 ug/ml herring sperm DNA) was added and reactions were incubated on ice for 15 minutes.

    Article Title: CD8A-binding fibronectin type III domains
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 pg of library DNA were incubated with 0.1 mM complete amino acids, 1× S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains
    Article Snippet: Human and rabbit serum albumin purified from serum (Sigma-Aldrich, St. Louis, Mo.) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μt and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains and uses thereof
    Article Snippet: Recombinant human and cyno serum albumin and human albumin domain II (Albumin Biosciences) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, IL), 100 mg/ml Herring Sperm DNA (Promega, Madison, WI), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, MO)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains
    Article Snippet: Human and rabbit serum albumin purified from serum (Sigma-Aldrich, St. Louis, Mo.) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1× S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Chimeric antigen receptors comprising BCMA-specific fibronectin type III domains and uses thereof
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution (lx TBS pH 7.4, 2% BSA, 100 ug/ml herring sperm DNA) were added and the reactions were incubated on ice for 15 minutes. .. The ITT reactions were incubated with biotinylated recombinant BCMA protein fused to the Fc domain of mouse IgG2a (AB Biosciences, catalog #P011F).

    Incubation:

    Article Title: Prostate specific membrane antigen binding fibronectin type III domains
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated at 30° C. with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL. .. After 1 hour, 375 μL of blocking solution (1×TBS pH 7.4, 0.01% I-block (Life Technologies, # T2015), 100 ug/ml herring sperm DNA) was added and reactions were incubated on ice for 15 minutes.

    Article Title: CD8A-binding fibronectin type III domains
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 pg of library DNA were incubated with 0.1 mM complete amino acids, 1× S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains
    Article Snippet: Human and rabbit serum albumin purified from serum (Sigma-Aldrich, St. Louis, Mo.) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μt and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains and uses thereof
    Article Snippet: Recombinant human and cyno serum albumin and human albumin domain II (Albumin Biosciences) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, IL), 100 mg/ml Herring Sperm DNA (Promega, Madison, WI), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, MO)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains
    Article Snippet: Human and rabbit serum albumin purified from serum (Sigma-Aldrich, St. Louis, Mo.) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1× S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Chimeric antigen receptors comprising BCMA-specific fibronectin type III domains and uses thereof
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution (lx TBS pH 7.4, 2% BSA, 100 ug/ml herring sperm DNA) were added and the reactions were incubated on ice for 15 minutes. .. The ITT reactions were incubated with biotinylated recombinant BCMA protein fused to the Fc domain of mouse IgG2a (AB Biosciences, catalog #P011F).

    Blocking Assay:

    Article Title: CD8A-binding fibronectin type III domains
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 pg of library DNA were incubated with 0.1 mM complete amino acids, 1× S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains
    Article Snippet: Human and rabbit serum albumin purified from serum (Sigma-Aldrich, St. Louis, Mo.) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μt and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains and uses thereof
    Article Snippet: Recombinant human and cyno serum albumin and human albumin domain II (Albumin Biosciences) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, IL), 100 mg/ml Herring Sperm DNA (Promega, Madison, WI), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, MO)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Serum albumin-binding fibronectin type III domains
    Article Snippet: Human and rabbit serum albumin purified from serum (Sigma-Aldrich, St. Louis, Mo.) was biotinylated using standard methods and used for panning. .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1× S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution ((0.1% Casein (Thermo Fisher, Rockford, Ill.), 100 mg/ml Herring Sperm DNA (Promega, Madison, Wis.), 1 mg/mL heparin (Sigma-Aldrich, St. Louis, Mo.)) was added and the reaction was incubated on ice for 15 minutes. ..

    Article Title: Chimeric antigen receptors comprising BCMA-specific fibronectin type III domains and uses thereof
    Article Snippet: .. For in vitro transcription and translation (ITT), 3 μg of library DNA were incubated with 0.1 mM complete amino acids, 1×S30 premix components, and 15 μL of S30 extract (Promega) in a total volume of 50 μL and incubated at 30° C. After 1 hour, 375 μL of blocking solution (lx TBS pH 7.4, 2% BSA, 100 ug/ml herring sperm DNA) were added and the reactions were incubated on ice for 15 minutes. .. The ITT reactions were incubated with biotinylated recombinant BCMA protein fused to the Fc domain of mouse IgG2a (AB Biosciences, catalog #P011F).

    Luciferase:

    Article Title: Silyl monomers capable of multimerizing in an aqueous solution, and methods of using same
    Article Snippet: Each reaction uses 2 μl (250 ng/μl) of the pBESTlucTM DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 al. Assays were carried out in Costar 96 well white round bottom plates. .. Each reaction uses 2 μl (250 ng/μl) of the pBESTluc™ DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 al. Assays were carried out in Costar 96 well white round bottom plates. .. Plates were incubated at 37° C. for one hour followed by addition of 35 μl of the Bright-Glo Luciferase Reagent (Promega Catalog #E2620).

    Article Title: Monomers capable of dimerizing in an aqueous solution, and methods of using same
    Article Snippet: .. Each reaction uses 2 μl (250 ng/μl) of the pBESTluc™ DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 μl of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 μl. .. Each reaction uses 2 μl (250 ng/μl) of the pBESTlucTM DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 μl of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 μl.

    Plasmid Preparation:

    Article Title: Silyl monomers capable of multimerizing in an aqueous solution, and methods of using same
    Article Snippet: Each reaction uses 2 μl (250 ng/μl) of the pBESTlucTM DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 al. Assays were carried out in Costar 96 well white round bottom plates. .. Each reaction uses 2 μl (250 ng/μl) of the pBESTluc™ DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 al. Assays were carried out in Costar 96 well white round bottom plates. .. Plates were incubated at 37° C. for one hour followed by addition of 35 μl of the Bright-Glo Luciferase Reagent (Promega Catalog #E2620).

    Article Title: Monomers capable of dimerizing in an aqueous solution, and methods of using same
    Article Snippet: .. Each reaction uses 2 μl (250 ng/μl) of the pBESTluc™ DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 μl of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 μl. .. Each reaction uses 2 μl (250 ng/μl) of the pBESTlucTM DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 μl of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 μl.

    Concentration Assay:

    Article Title: Silyl monomers capable of multimerizing in an aqueous solution, and methods of using same
    Article Snippet: Each reaction uses 2 μl (250 ng/μl) of the pBESTlucTM DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 al. Assays were carried out in Costar 96 well white round bottom plates. .. Each reaction uses 2 μl (250 ng/μl) of the pBESTluc™ DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 al. Assays were carried out in Costar 96 well white round bottom plates. .. Plates were incubated at 37° C. for one hour followed by addition of 35 μl of the Bright-Glo Luciferase Reagent (Promega Catalog #E2620).

    Article Title: Monomers capable of dimerizing in an aqueous solution, and methods of using same
    Article Snippet: .. Each reaction uses 2 μl (250 ng/μl) of the pBESTluc™ DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 μl of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 μl. .. Each reaction uses 2 μl (250 ng/μl) of the pBESTlucTM DNA based circular luciferase plasmid (Promega Catalog #L492A), with 4 μl of complete amino acid mix (Promega Catalog #L4461), 13 μl of S30 Premix Without Amino Acids (Promega Catalog #L512A), 5 μl of S30 Extract (Promega Catalog #L464A), monomers at the appropriate concentration, and nuclease free water in a total volume of 35 μl.



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